Toluleke Famuyiwa
Florida Atlantic University, USA
Title: ABC transporters mediated multidrug resistance in prostate cancer cells
Biography
Biography: Toluleke Famuyiwa
Abstract
Background: Prostate cancer is the second most diagnosed cancer. This study focuses on overcoming ATP Binding Cassette (ABC)-mediated drug resistance in prostate cancer treatment. The objective of study: This study aims to (i) investigate the interaction between 3-BPA and SC-514, (ii) reduce treatmentinduced ABC-mediated Multidrug Resistance(MDR), and (iii) investigate the signaling pathways involved in ABC transportermediated MDR. Method: We utilized Poly Lactic-co-Glycolic Acid (PLGA) nanoparticles as a co-delivery system for SC-514 and 3-Bromopyruvate (3-BPA) in LNCaP cells. The impact of varying concentrations of these drugs on LNCaP cells was studied. Bioassays used included Trypan Blue, MTT, and NBT. Fluorescence microscopy was performed. Results: A One-way ANOVA was conducted to compare 3-BPA, SC-514, and the combination of 3-BPA and SC-514 after 24 hours of treatment. The result shows that the p-value= 0.00023. Regression analysis of the results from the time-dependent experiments was performed. The regression analysis showed the following p-values: 24hrs (0.00023), 48hrs (0.00003), 72hrs (0.000000152), 96hrs (0.000000049). ROS levels of LNCaP cells treated with 3-BPA (r=-0.5), SC-514 (r=- 0.72) and 3-BPA + SC-514 (r=-0.58) were compared using one-way ANOVA. The result showed no significant difference in ROS modulation (p=0.54). Conclusion: There is a weak to moderate correlation between ROS levels and cell death. Additionally, there was a positive correlation between the drug concentrations and cell death.